Session 3 - Analytical Aspects of D-Dimer Assays

Join our third session on the Analytical Aspects of D-Dimer Assays with Miquel Sales!


About this webinar


D-dimer molecules are generated through the degradation of cross-linked fibrin during fibrinolysis and require the activity of thrombin, activated factor XIII (factor XIIIa), and plasmin. The action of Plasmin on the cross-linked fibrin clot results in the D-dimer molecule. The D-dimer assays have extensively been used and studied in the clinical management of Venous Thromboembolism for the exclusion of Deep Vein Thrombosis or Pulmonary Embolism, using a test specific cutoff value, units (D-Dimer Units or Fibrinogen Equivalent Unit), sensitivity, specificity, and negative predictive value.
D-dimer can be tested on whole blood, plasma, or serum using antibodies that recognize a specific epitope on cross-linked D-dimer molecules. There are many commercially available D-dimer assays thatare available using either enzyme-linked immunosorbent assays (ELISA), immunofluorescent assays, latex agglutination assays, or chemiluminescent assays. Each of these test methods differs in its limitations and interferences.
Laboratory assays are validated with standard, however, for D-Dimer the lack of such a single standard makes a direct comparison of different assays challenging. Since manufacturers use different antibodies that recognize different epitopes with varying kinetics makes the development of a universal standard for calibration a difficult project which makes standardization unlikely. Please join us for the talk by Miquel Sales who provides a comprehensive overview of the analytical aspects of D-Dimer Assays.

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